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PCR/qPCR
Polymerase chain reaction related questions
How to use passive PCR reference dye?This is a short description of the best practice to use ROX ref. dye in PCR experiments- Recommendations for primers and probes designUser guidelines for selecting primers and probes for polymerase chain reaction (PCR)
- Oligonucleotides delivered! What's next?Here, we describe how to prepare stock solutions of Lumiprobe's oligonucleotides
Quantitative real-time PCR with dsGreen?Advantages of using dsGreen dye in qPCR and comparison with other fluorescent dyes: EvaGreen® dye and TaqMan® probes
Are there any differences between probes containing 5-JOE and 6-JOE isomers in probe-based qPCR?qPCR performed with probes containing either 5- or 6-isomer of JOE has shown no significant differences between isomers