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Protein labeling
Protein labeling related questions
Do you have a protocol for labeling proteins with hydrazide-based dyes?Brief protocol for protein labeling using hydrazide functionalized dyes.
Do the sulfated groups in Cyanine dyes stand up to the rigors of the trifluoroacetic acid cleavage step of the peptide from the resin?Stability of sulfonated Cyanine dyes in concentrated trifluoroacetic acid (TFA) during solid phase peptide synthesis (SPPS).- Is it possible to label Tyrosine-containing peptides?Labeling of the Tyr-containing peptides is possible according to the literature.
Conjugation of antibodies with DBCO and downstream copper-free click chemistry reactionCopper-free click chemistry conjugation guidelines, considerations, and reaction scheme.
How do I calculate the optimal amounts and procedure to label protein/antibody?This article is about a fast and simple Lumiprobe labeling calculator that gives you optimal amounts and procedure of AB labeling.
Is it possible to label dilute antibodies with the Lumiprobe ready-to-use kits?Even when the concentration of Ab in the solution is below the recommended concentration of 1 mg/mL, it is still possible to use the Lumiprobe antibody labeling kits to achieve a labeling degree of 2-3 dye molecules per Ab.
Why do I see the precipitates after protein labeling with non-sulfonated dyes followed by dialysis?Non-sulfonated versus sulfonated dyes for proteins labeling with Lumiprobe dyes.
How much of dye is in each reaction sulfo-Cyanine3 antibody labeling kit?Amount of dye per reaction in sulfo-Cyanine3 antibody labeling kit- Is it possible to label peptides with pyrilium dyes?Step-by-step description of our experience labeling peptides with Pyrilium-1 (Py-1).
How to attach DBCO (ADIBO) group to an antibody?Description of a short experiment we have performed to introduce DBCO label into a mouse monoclonal antibody.