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Customers' Q&A
All interesting content we have reported to our customers following their requests.
- Is it feasible to use sulfo-Cyanine maleimide labels in distilled water?Using distilled water for maleimide labeling.
Is there any concern about a reduction of fluorescence intensity by epoxidation of Cyanine sequent double bonds during preparation and after the dye is released from nanoparticles in vivo? Does fluorescence intensity remain or get weak by epoxidation?Epoxidation of Cyanine dyes.
Does the fluorescence of Cyanine dyes depend on pH?The fluorescence dependency of Cyanine dyes such as Cyanine3, sulfo-Cyanine3, Cyanine5, sulfo-Cyanine5, and boron-dipyrromethene dyes such as BDP TMR, BDP 630/650 on pH conditions.- Why my xanthene amidites and supports does not fluoresce?Xanthene amidites and supports (FAM, TET, HEX, JOE, SIMA, R6G, VIC-derivatives) have data on fluorescence with ~500/530 excitation/emission maxima, but all products are colourless or slightly coloured substances. How is it possible?
I would like to label tyramine molecule with a fluorescent dye. Is it possible?The protocol for labeling of tyramine with NHS ester functionalized Cyanine dye for use in tyramide signal amplification.
What is the solubility of Cyanine7 NHS ester and sulfo-Cyanine7 NHS ester in ethanol, diethyl ether, acetone, and dichloromethane?The solubility of Cyanine7 derivatives in different organic solvents.- What are hydrolysis rates for NHS ester functionalized dyes?NHS ester tends to hydrolyze over time in the water.
- Naming of PEGs by LumiprobeAbout how we name our PEG products and why our PEG3 products are PEG2 in other manufacturers and vice versa
Are NIR dyes visible in the daylight?Is there any visible color change when excited?
What is the solubility of BDP FL hydrazide in water?The water solubility of BDP FL hydrazide dye in water- I’ve been using your Cyanine7.5 amine to label some of my polymers, but I can’t seem to get any signal when I test the fluorescence on the fluorometer.Possible reasons why you don't see any fluorescence signal from conjugates or the dye alone or in different solvent systems.
We attempted to attach sulfo-Cyanine7 NHS ester to modified triphosphate, but the resultant product had really low fluorescence.Low fluorescence of sulfo-Cyanine7 conjugated to triphosphate measured by Agilent 1260 Infinity Fluorescence Detector.
What is the proper color of Cyanine5 succinimidyl ester?This article is to explain the possible appearance and color range you may observe for Cyanine 5 NHS ester powder/thin films.- What are the solubility of sulfo-Cyanine7 amine and Cyanine7 amine in water?The water solubility of Cyanine7 amine derivatives.
- Any suggestions for FRET pair of dyes?FRET pairs of dyes we can recommend from our catalog products
- What is the color of sulfo-Cyanine7.5 NHS ester after dissolving in water and in DMF?The color of sulfo-Cyanine7.5 NHS ester in water and in DMF solutions.
What is the solubility of Cyanine7.5 amine in water?The water solubility of Cyanine7.5 amine product.- Why are Cyanine dyes having low quantum yields still widely used?The quantum yield of Cyanine dyes.
Are Cyanine dyes stable in 0.1-0.5% bleach solution?Stability of Cyanine dyes in a bleach solution.