Центр помощи
Ответы на частые вопросы о наших реагентах и их применении.
Недавно обновлённые
- Customers' Q&AIs it feasible to use sulfo-Cyanine maleimide labels in distilled water?
- Biomolecule quantificationWhat is the structure of the dye in the PreciseGreen DNA quantification solution?
- Biomolecule quantificationDo you have any data to show how good it is for PreciseGreen to bind the dsDNA than the ssDNA?
Темы
Customers' Q&A
All interesting content we have reported to our customers following their requests.
- Is it feasible to use sulfo-Cyanine maleimide labels in distilled water?
- Is there any concern about a reduction of fluorescence intensity by epoxidation of Cyanine sequent double bonds during preparation and after the dye is released from nanoparticles in vivo? Does fluorescence intensity remain or get weak by epoxidation?
- Does the fluorescence of Cyanine dyes depend on pH?
- Why my xanthene amidites and supports does not fluoresce?
- I would like to label tyramine molecule with a fluorescent dye. Is it possible?
PCR/qPCR
Polymerase chain reaction related questions
Staining cells
Questions related to staining of live and fixed cells with Lumiprobe ready-to-use kits and dyes
- Are CytoTracer® dyes applicable to plant cell staining?
- Can I use Lumiprobe's dyes for two-photon microscopy? What excitation and emission wavelengths do they have?
- Staining lipid droplets with BDP® 493/503 lipid stain
- Staining of cells with Fe(II)-selective fluorescent probe HMRhoNox-M
- Can exosomes be stained with PKH26?
Protein labeling
Protein labeling related questions
- Do you have a protocol for labeling proteins with hydrazide-based dyes?
- Do the sulfated groups in Cyanine dyes stand up to the rigors of the trifluoroacetic acid cleavage step of the peptide from the resin?
- Is it possible to label Tyrosine-containing peptides?
- Conjugation of antibodies with DBCO and downstream copper-free click chemistry reaction
- How do I calculate the optimal amounts and procedure to label protein/antibody?
Click chemistry
Click chemistry related questions
Electrophoresis
Electrophoresis and gel staining related questions
- How to perform DNA Electrophoresis in Agarose Gel and stain the Gel with dsGreen?
- What does "minimal" mean in Cyanine NHS ester minimal dye products, and can Cyanine2, Cyanine3, and Cyanine5 be mixed all together?
- What is the best practice of electrophoresis gel staining with dsGreen?
- A guide for visualization of gels stained with dsGreen using the Gel Doc™ EZ System.
- Staining proteins with ProteOrange in polyacrylamide gels.
Biomolecule quantification
Questions related to protein and nucleic acid quantification
- What is the structure of the dye in the PreciseGreen DNA quantification solution?
- Do you have any data to show how good it is for PreciseGreen to bind the dsDNA than the ssDNA?
- How to perform the measurements with a fluorometer using QuDye Protein Quantification kit?
- Quantification in 96-well Fluorescent Microplate reader
- Is it possible to use the PreciseGreen DNA quantification solution or kit with the most popular reader?